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Measurements of Homogentisic Acid levels in Alkaptonuria Patients Using an Optimized and Validated Gas Chromatography Method/Mass Spectrometry
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نوع المصدر |
مقال
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بيانات التأليف |
Al-Sarayreh, Sameeh A. (Author)
Al-Tarawneh, Ibrahim N. (Author)
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بيانات الدورية المصدر |
العنوان:
Jordan journal of biological sciences
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رقم المجلد/ العدد: 2014/DEC V.7 N.4
رقم الإستدعاء: 570.5 J J B الموقع: Periodicals & References Hall - 2nd floor
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الوصف المادي |
p 195-198
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رأس الموضوع/ الواصفات |
Life sciences (Biology)
(1295)
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المستخلص |
Alkaptonuria is a very rare genetic disorder, characterized by a lack of homogentisate dioxygenase and causes
accumulation of homogentisic acid. Clinical manifestations include dark urine, dark-black pigmentation of connective
tissues (ochronosis), and arthritis of large joints and spine. The disease is usually diagnosed in adulthood by assessment of
signs and symptoms of ochronosis. Confirmation of suspected diagnosis can be achieved by quantitative measurements of
homogentisic acid levels. In this study, homogentisic acid was analyzed in 17 alkaptonuria patients. After liquid-liquid
extraction, the analyte was determined by Gas Chromatography/ Mass Spectrometry (GC-MS) method. For quantitation
purposes, external calibration was applied first, regression coefficient of = 0.995 indicated the linearity in the concentration
range of 1-100 ng/µl. The instrumental detection limit (IDL) and lower limit of quantitation were 3.82 and 12.7 µg/L,
respectively. Recovery rate was = 89%. Precision given ...
المستخلص الكامل
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| المزيد من المقالات من
الدورية:
Jordan journal of biological sciences
(44)
العدد: 2014/DEC V.7 N.4
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إبحث في المصادر الإلكترونية |
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مواضيع ذات صلة |
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